human escc cell lines  (ATCC)

Journal: BMC Cancer

Article Title: Role of Cancer Associated Fibroblast (CAF) derived miRNAs on head and neck malignancies microenvironment: a systematic review

doi: 10.1186/s12885-025-13965-9

Figure Lengend Snippet: Summary characteristics of included studies

Article Snippet: Khazaei et al. [ ] , 2017 , Iran , In vitro (Human) , esophageal squamous cell carcinoma (ESCC) , Case sample: We gathered 26 sets of fresh-frozen tissues and 39 blood serum samples from patients diagnosed with esophageal squamous cell carcinoma (ESCC) at Sayad Shirazi Hospital, part of Golestan University of Medical Sciences in Iran The KYSE-30 cell line (human ESCC) and HFSF-PI3 cell line (human normal fibroblasts from skin) were obtained from the National Cell Bank of Iran (Pasteur Institute) , 1. Isolation of RNA from esophageal tissue, serum samples, and purified exosomes Quantification of miR-451 using quantitative reverse transcription polymerase chain reaction (RT-PCR) 3. Exosome isolation 4. Cell culture conditions and miR-451 overexpression 5. Co-cultivation of an esophageal cancer cell line with normal fibroblasts (HFSF-P13) 6. Migration Analysis Analyzing MIF expression in the co-cultured cell lines 8. Statistical analyses , • Serum samples from patients with esophageal cancer exhibited elevated levels of miR-451. Subsequent analysis of cryopreserved tumor tissues from the same patients revealed reduced miR-451 expression • When the KYSE-30 cell line was co-cultured with normal fibroblasts, there was a notable increase in the release of miR-451 exosomes into the culture medium. When the KYSE-30 cell line was co-cultured with fibroblasts expressing high amounts of miR-451, there was a significant increase in the migratory capacity of the KYSE-30 cells. Line • The MIF expression in the KYSE-30 cell line showed an increase, although it was not statistically significant. MIF is a confirmed target of miR-451 , • The study shows that cancer-associated fibroblasts use exosomal miR-451 as a signaling molecule to promote tumor cell migration and accelerate cancer progression.

Techniques: In Vitro, Control, Formalin-fixed Paraffin-Embedded, Expressing, Quantitative RT-PCR, Cell Culture, Transfection, Western Blot, Migration, Biomarker Discovery, Isolation, Purification, Reverse Transcription, Polymerase Chain Reaction, Over Expression, Labeling, Transmission Assay, Electron Microscopy, RNA Extraction, Sequencing, Quantitative Proteomics, Real-time Polymerase Chain Reaction, Immunofluorescence, Immunohistochemistry, Luciferase, Gene Expression, Disruption, Reporter Assay, Immunodepletion, Amplification, Immunocytochemistry, Flow Cytometry, Derivative Assay, Immunohistochemical staining, Extraction, Microarray, Contraction Assay, Comparison, In Vivo, Ex Vivo, Staining, Invasion Assay, Tube Formation Assay, Fluorescence, In Situ Hybridization, Reverse Transcription Polymerase Chain Reaction, Inhibition, Apoptosis Assay, Enzyme-linked Immunosorbent Assay, Produced, Phospho-proteomics, Transformation Assay, Activation Assay, Cell Isolation, Transduction, Stable Transfection, DNA Methylation Assay, Cell Migration Assay, Plasmid Preparation, Zymography, Functional Assay, Translocation Assay, Clinical Proteomics, Construct, MTT Assay, Co-culture Assay, Pull Down Assay, Immunoprecipitation, Tumorigenicity Assay, Transferring, RNA Sequencing, Knockdown, Small Interfering RNA, Immunostaining, Protein-Protein interactions, Gradient Centrifugation, CCK-8 Assay, Injection, Protein Extraction